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plix403 puromycin david root addgene plasmid  (Addgene inc)


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    Addgene inc plix403 puromycin david root addgene plasmid
    Plix403 Puromycin David Root Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plix403+puromycin+david+root+addgene+plasmid/pLIX_403+(Plasmid+%2341395)/pm40412389-1017-272-276
    Average 96 stars, based on 123 article reviews
    plix403 puromycin david root addgene plasmid - by Bioz Stars, 2026-10
    96/100 stars

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    RNA Sequencing:

    Article Title: Structural basis for OAS2 regulation and its antiviral function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Human OAS2 This paper PDB: 9H1Z Human OAS2 map This paper EMDB: EMD-51786 RNA-seq This paper ArrayExpress: E-MTAB-14791 Human OAS2-RNA MD Simulation This paper https://doi.org/10.5281/zenodo.14753364 Unprocessed WB and microscopy data This paper https://doi.org/10.17632/tvgpf9t2jm.1 RNA-seq dataset THP-1 cells with OAS2 MIS-C mutations Lee et al.34 NCBI-SRA: PRJNA898284 Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 HEK293T RNASEL KO monoclonal cells This paper N/A HEK293T NTC KO monoclonal cells This paper N/A A549 Georg Kochs N/A Primary BJ HFF Andreas Pichlmair lab, Huang et al.91 N/A A549 OAS2 KO This paper N/A A549 NTC KO This paper N/A A549 OAS2 KO OAS2 WT KI cells This paper N/A A549 OAS2 KO OAS2 C652S KI cells This paper N/A A549 OAS2 KO OAS2 G2A KI cells This paper N/A A549 OAS2 KO OAS2 G2A C652S KI cells This paper N/A A549 OAS2 KO OAS2 F524L KI cells This paper N/A HeLa H1 ATCC Cat# CRL-1958 BJ HFF hTert+ Andreas Pichlmair, Huang et al.91 N/A Vero E6/TMPRSS2 Dr. Makoto Takeda, National Institute for Biological Standards and Control (NIBSC) Cat#100978 Experimental models: Organisms/strains Trichoplusia ni cells Expression Systems N/A Spodoptera frugiperda sf9 cells Expression Systems N/A Oligonucleotides See Table S2 N/A Recombinant DNA OAS plasmid constructs see Table S2 This paper N/A pET-28M-SUMO1-human OAS1 p46 This paper N/A pET-28M-SUMO1-human OAS1 p42 This paper N/A pACEBac1_N-6xHis_3C_hOAS3 p100 This paper N/A 2′5′-OA biosensor RNase L-Luciferase plasmid Alexei Korennykh, Chitrakar et al.62 N/A pACEBac1_N-6xHis_3C_hOAS2 p71 This paper N/A pACEBac1_N-6xHis_3C_hOAS2 p69 This paper N/A pACEBac1_N-6xHis_3C_hOAS2-C652S This paper N/A pACEBac1_N-6xHis_3C_hOAS2-p71 FL D153A S150A This paper N/A pLentiCRISPRv2 (hygromycin) Stringer et al. (2019)92 Addgene plasmid # 98291 pLIX403 (puromycin) David Root Addgene plasmid #41395 pLenti CMV/TO Zeo_GW (zeocin) Campeau et al. (2009)93 Addgene plasmid # 17294 pMD2-VSVG Andreas Pichlmair89 N/A pCMV-Gag-Pol Andreas Pichlmair89 N/A pCG_ACE2_IRES_eGFP Xie et al. (2023)94 N/A pCR XL TOPO OC43 N fragment This paper N/A pCR XL TOPO NL63 N fragment This paper N/A (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 85, 1–18.e1–e13, June 5, 2025 e3

    Western Blot:

    Article Title: Structural basis for OAS2 regulation and its antiviral function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Human OAS2 This paper PDB: 9H1Z Human OAS2 map This paper EMDB: EMD-51786 RNA-seq This paper ArrayExpress: E-MTAB-14791 Human OAS2-RNA MD Simulation This paper https://doi.org/10.5281/zenodo.14753364 Unprocessed WB and microscopy data This paper https://doi.org/10.17632/tvgpf9t2jm.1 RNA-seq dataset THP-1 cells with OAS2 MIS-C mutations Lee et al.34 NCBI-SRA: PRJNA898284 Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 HEK293T RNASEL KO monoclonal cells This paper N/A HEK293T NTC KO monoclonal cells This paper N/A A549 Georg Kochs N/A Primary BJ HFF Andreas Pichlmair lab, Huang et al.91 N/A A549 OAS2 KO This paper N/A A549 NTC KO This paper N/A A549 OAS2 KO OAS2 WT KI cells This paper N/A A549 OAS2 KO OAS2 C652S KI cells This paper N/A A549 OAS2 KO OAS2 G2A KI cells This paper N/A A549 OAS2 KO OAS2 G2A C652S KI cells This paper N/A A549 OAS2 KO OAS2 F524L KI cells This paper N/A HeLa H1 ATCC Cat# CRL-1958 BJ HFF hTert+ Andreas Pichlmair, Huang et al.91 N/A Vero E6/TMPRSS2 Dr. Makoto Takeda, National Institute for Biological Standards and Control (NIBSC) Cat#100978 Experimental models: Organisms/strains Trichoplusia ni cells Expression Systems N/A Spodoptera frugiperda sf9 cells Expression Systems N/A Oligonucleotides See Table S2 N/A Recombinant DNA OAS plasmid constructs see Table S2 This paper N/A pET-28M-SUMO1-human OAS1 p46 This paper N/A pET-28M-SUMO1-human OAS1 p42 This paper N/A pACEBac1_N-6xHis_3C_hOAS3 p100 This paper N/A 2′5′-OA biosensor RNase L-Luciferase plasmid Alexei Korennykh, Chitrakar et al.62 N/A pACEBac1_N-6xHis_3C_hOAS2 p71 This paper N/A pACEBac1_N-6xHis_3C_hOAS2 p69 This paper N/A pACEBac1_N-6xHis_3C_hOAS2-C652S This paper N/A pACEBac1_N-6xHis_3C_hOAS2-p71 FL D153A S150A This paper N/A pLentiCRISPRv2 (hygromycin) Stringer et al. (2019)92 Addgene plasmid # 98291 pLIX403 (puromycin) David Root Addgene plasmid #41395 pLenti CMV/TO Zeo_GW (zeocin) Campeau et al. (2009)93 Addgene plasmid # 17294 pMD2-VSVG Andreas Pichlmair89 N/A pCMV-Gag-Pol Andreas Pichlmair89 N/A pCG_ACE2_IRES_eGFP Xie et al. (2023)94 N/A pCR XL TOPO OC43 N fragment This paper N/A pCR XL TOPO NL63 N fragment This paper N/A (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 85, 1–18.e1–e13, June 5, 2025 e3

    Microscopy:

    Article Title: Structural basis for OAS2 regulation and its antiviral function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Human OAS2 This paper PDB: 9H1Z Human OAS2 map This paper EMDB: EMD-51786 RNA-seq This paper ArrayExpress: E-MTAB-14791 Human OAS2-RNA MD Simulation This paper https://doi.org/10.5281/zenodo.14753364 Unprocessed WB and microscopy data This paper https://doi.org/10.17632/tvgpf9t2jm.1 RNA-seq dataset THP-1 cells with OAS2 MIS-C mutations Lee et al.34 NCBI-SRA: PRJNA898284 Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 HEK293T RNASEL KO monoclonal cells This paper N/A HEK293T NTC KO monoclonal cells This paper N/A A549 Georg Kochs N/A Primary BJ HFF Andreas Pichlmair lab, Huang et al.91 N/A A549 OAS2 KO This paper N/A A549 NTC KO This paper N/A A549 OAS2 KO OAS2 WT KI cells This paper N/A A549 OAS2 KO OAS2 C652S KI cells This paper N/A A549 OAS2 KO OAS2 G2A KI cells This paper N/A A549 OAS2 KO OAS2 G2A C652S KI cells This paper N/A A549 OAS2 KO OAS2 F524L KI cells This paper N/A HeLa H1 ATCC Cat# CRL-1958 BJ HFF hTert+ Andreas Pichlmair, Huang et al.91 N/A Vero E6/TMPRSS2 Dr. Makoto Takeda, National Institute for Biological Standards and Control (NIBSC) Cat#100978 Experimental models: Organisms/strains Trichoplusia ni cells Expression Systems N/A Spodoptera frugiperda sf9 cells Expression Systems N/A Oligonucleotides See Table S2 N/A Recombinant DNA OAS plasmid constructs see Table S2 This paper N/A pET-28M-SUMO1-human OAS1 p46 This paper N/A pET-28M-SUMO1-human OAS1 p42 This paper N/A pACEBac1_N-6xHis_3C_hOAS3 p100 This paper N/A 2′5′-OA biosensor RNase L-Luciferase plasmid Alexei Korennykh, Chitrakar et al.62 N/A pACEBac1_N-6xHis_3C_hOAS2 p71 This paper N/A pACEBac1_N-6xHis_3C_hOAS2 p69 This paper N/A pACEBac1_N-6xHis_3C_hOAS2-C652S This paper N/A pACEBac1_N-6xHis_3C_hOAS2-p71 FL D153A S150A This paper N/A pLentiCRISPRv2 (hygromycin) Stringer et al. (2019)92 Addgene plasmid # 98291 pLIX403 (puromycin) David Root Addgene plasmid #41395 pLenti CMV/TO Zeo_GW (zeocin) Campeau et al. (2009)93 Addgene plasmid # 17294 pMD2-VSVG Andreas Pichlmair89 N/A pCMV-Gag-Pol Andreas Pichlmair89 N/A pCG_ACE2_IRES_eGFP Xie et al. (2023)94 N/A pCR XL TOPO OC43 N fragment This paper N/A pCR XL TOPO NL63 N fragment This paper N/A (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 85, 1–18.e1–e13, June 5, 2025 e3

    Control:

    Article Title: Structural basis for OAS2 regulation and its antiviral function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Human OAS2 This paper PDB: 9H1Z Human OAS2 map This paper EMDB: EMD-51786 RNA-seq This paper ArrayExpress: E-MTAB-14791 Human OAS2-RNA MD Simulation This paper https://doi.org/10.5281/zenodo.14753364 Unprocessed WB and microscopy data This paper https://doi.org/10.17632/tvgpf9t2jm.1 RNA-seq dataset THP-1 cells with OAS2 MIS-C mutations Lee et al.34 NCBI-SRA: PRJNA898284 Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 HEK293T RNASEL KO monoclonal cells This paper N/A HEK293T NTC KO monoclonal cells This paper N/A A549 Georg Kochs N/A Primary BJ HFF Andreas Pichlmair lab, Huang et al.91 N/A A549 OAS2 KO This paper N/A A549 NTC KO This paper N/A A549 OAS2 KO OAS2 WT KI cells This paper N/A A549 OAS2 KO OAS2 C652S KI cells This paper N/A A549 OAS2 KO OAS2 G2A KI cells This paper N/A A549 OAS2 KO OAS2 G2A C652S KI cells This paper N/A A549 OAS2 KO OAS2 F524L KI cells This paper N/A HeLa H1 ATCC Cat# CRL-1958 BJ HFF hTert+ Andreas Pichlmair, Huang et al.91 N/A Vero E6/TMPRSS2 Dr. Makoto Takeda, National Institute for Biological Standards and Control (NIBSC) Cat#100978 Experimental models: Organisms/strains Trichoplusia ni cells Expression Systems N/A Spodoptera frugiperda sf9 cells Expression Systems N/A Oligonucleotides See Table S2 N/A Recombinant DNA OAS plasmid constructs see Table S2 This paper N/A pET-28M-SUMO1-human OAS1 p46 This paper N/A pET-28M-SUMO1-human OAS1 p42 This paper N/A pACEBac1_N-6xHis_3C_hOAS3 p100 This paper N/A 2′5′-OA biosensor RNase L-Luciferase plasmid Alexei Korennykh, Chitrakar et al.62 N/A pACEBac1_N-6xHis_3C_hOAS2 p71 This paper N/A pACEBac1_N-6xHis_3C_hOAS2 p69 This paper N/A pACEBac1_N-6xHis_3C_hOAS2-C652S This paper N/A pACEBac1_N-6xHis_3C_hOAS2-p71 FL D153A S150A This paper N/A pLentiCRISPRv2 (hygromycin) Stringer et al. (2019)92 Addgene plasmid # 98291 pLIX403 (puromycin) David Root Addgene plasmid #41395 pLenti CMV/TO Zeo_GW (zeocin) Campeau et al. (2009)93 Addgene plasmid # 17294 pMD2-VSVG Andreas Pichlmair89 N/A pCMV-Gag-Pol Andreas Pichlmair89 N/A pCG_ACE2_IRES_eGFP Xie et al. (2023)94 N/A pCR XL TOPO OC43 N fragment This paper N/A pCR XL TOPO NL63 N fragment This paper N/A (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 85, 1–18.e1–e13, June 5, 2025 e3

    Recombinant:

    Article Title: Structural basis for OAS2 regulation and its antiviral function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Human OAS2 This paper PDB: 9H1Z Human OAS2 map This paper EMDB: EMD-51786 RNA-seq This paper ArrayExpress: E-MTAB-14791 Human OAS2-RNA MD Simulation This paper https://doi.org/10.5281/zenodo.14753364 Unprocessed WB and microscopy data This paper https://doi.org/10.17632/tvgpf9t2jm.1 RNA-seq dataset THP-1 cells with OAS2 MIS-C mutations Lee et al.34 NCBI-SRA: PRJNA898284 Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 HEK293T RNASEL KO monoclonal cells This paper N/A HEK293T NTC KO monoclonal cells This paper N/A A549 Georg Kochs N/A Primary BJ HFF Andreas Pichlmair lab, Huang et al.91 N/A A549 OAS2 KO This paper N/A A549 NTC KO This paper N/A A549 OAS2 KO OAS2 WT KI cells This paper N/A A549 OAS2 KO OAS2 C652S KI cells This paper N/A A549 OAS2 KO OAS2 G2A KI cells This paper N/A A549 OAS2 KO OAS2 G2A C652S KI cells This paper N/A A549 OAS2 KO OAS2 F524L KI cells This paper N/A HeLa H1 ATCC Cat# CRL-1958 BJ HFF hTert+ Andreas Pichlmair, Huang et al.91 N/A Vero E6/TMPRSS2 Dr. Makoto Takeda, National Institute for Biological Standards and Control (NIBSC) Cat#100978 Experimental models: Organisms/strains Trichoplusia ni cells Expression Systems N/A Spodoptera frugiperda sf9 cells Expression Systems N/A Oligonucleotides See Table S2 N/A Recombinant DNA OAS plasmid constructs see Table S2 This paper N/A pET-28M-SUMO1-human OAS1 p46 This paper N/A pET-28M-SUMO1-human OAS1 p42 This paper N/A pACEBac1_N-6xHis_3C_hOAS3 p100 This paper N/A 2′5′-OA biosensor RNase L-Luciferase plasmid Alexei Korennykh, Chitrakar et al.62 N/A pACEBac1_N-6xHis_3C_hOAS2 p71 This paper N/A pACEBac1_N-6xHis_3C_hOAS2 p69 This paper N/A pACEBac1_N-6xHis_3C_hOAS2-C652S This paper N/A pACEBac1_N-6xHis_3C_hOAS2-p71 FL D153A S150A This paper N/A pLentiCRISPRv2 (hygromycin) Stringer et al. (2019)92 Addgene plasmid # 98291 pLIX403 (puromycin) David Root Addgene plasmid #41395 pLenti CMV/TO Zeo_GW (zeocin) Campeau et al. (2009)93 Addgene plasmid # 17294 pMD2-VSVG Andreas Pichlmair89 N/A pCMV-Gag-Pol Andreas Pichlmair89 N/A pCG_ACE2_IRES_eGFP Xie et al. (2023)94 N/A pCR XL TOPO OC43 N fragment This paper N/A pCR XL TOPO NL63 N fragment This paper N/A (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 85, 1–18.e1–e13, June 5, 2025 e3

    Plasmid Preparation:

    Article Title: Structural basis for OAS2 regulation and its antiviral function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Human OAS2 This paper PDB: 9H1Z Human OAS2 map This paper EMDB: EMD-51786 RNA-seq This paper ArrayExpress: E-MTAB-14791 Human OAS2-RNA MD Simulation This paper https://doi.org/10.5281/zenodo.14753364 Unprocessed WB and microscopy data This paper https://doi.org/10.17632/tvgpf9t2jm.1 RNA-seq dataset THP-1 cells with OAS2 MIS-C mutations Lee et al.34 NCBI-SRA: PRJNA898284 Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 HEK293T RNASEL KO monoclonal cells This paper N/A HEK293T NTC KO monoclonal cells This paper N/A A549 Georg Kochs N/A Primary BJ HFF Andreas Pichlmair lab, Huang et al.91 N/A A549 OAS2 KO This paper N/A A549 NTC KO This paper N/A A549 OAS2 KO OAS2 WT KI cells This paper N/A A549 OAS2 KO OAS2 C652S KI cells This paper N/A A549 OAS2 KO OAS2 G2A KI cells This paper N/A A549 OAS2 KO OAS2 G2A C652S KI cells This paper N/A A549 OAS2 KO OAS2 F524L KI cells This paper N/A HeLa H1 ATCC Cat# CRL-1958 BJ HFF hTert+ Andreas Pichlmair, Huang et al.91 N/A Vero E6/TMPRSS2 Dr. Makoto Takeda, National Institute for Biological Standards and Control (NIBSC) Cat#100978 Experimental models: Organisms/strains Trichoplusia ni cells Expression Systems N/A Spodoptera frugiperda sf9 cells Expression Systems N/A Oligonucleotides See Table S2 N/A Recombinant DNA OAS plasmid constructs see Table S2 This paper N/A pET-28M-SUMO1-human OAS1 p46 This paper N/A pET-28M-SUMO1-human OAS1 p42 This paper N/A pACEBac1_N-6xHis_3C_hOAS3 p100 This paper N/A 2′5′-OA biosensor RNase L-Luciferase plasmid Alexei Korennykh, Chitrakar et al.62 N/A pACEBac1_N-6xHis_3C_hOAS2 p71 This paper N/A pACEBac1_N-6xHis_3C_hOAS2 p69 This paper N/A pACEBac1_N-6xHis_3C_hOAS2-C652S This paper N/A pACEBac1_N-6xHis_3C_hOAS2-p71 FL D153A S150A This paper N/A pLentiCRISPRv2 (hygromycin) Stringer et al. (2019)92 Addgene plasmid # 98291 pLIX403 (puromycin) David Root Addgene plasmid #41395 pLenti CMV/TO Zeo_GW (zeocin) Campeau et al. (2009)93 Addgene plasmid # 17294 pMD2-VSVG Andreas Pichlmair89 N/A pCMV-Gag-Pol Andreas Pichlmair89 N/A pCG_ACE2_IRES_eGFP Xie et al. (2023)94 N/A pCR XL TOPO OC43 N fragment This paper N/A pCR XL TOPO NL63 N fragment This paper N/A (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 85, 1–18.e1–e13, June 5, 2025 e3

    Construct:

    Article Title: Structural basis for OAS2 regulation and its antiviral function.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Human OAS2 This paper PDB: 9H1Z Human OAS2 map This paper EMDB: EMD-51786 RNA-seq This paper ArrayExpress: E-MTAB-14791 Human OAS2-RNA MD Simulation This paper https://doi.org/10.5281/zenodo.14753364 Unprocessed WB and microscopy data This paper https://doi.org/10.17632/tvgpf9t2jm.1 RNA-seq dataset THP-1 cells with OAS2 MIS-C mutations Lee et al.34 NCBI-SRA: PRJNA898284 Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 HEK293T RNASEL KO monoclonal cells This paper N/A HEK293T NTC KO monoclonal cells This paper N/A A549 Georg Kochs N/A Primary BJ HFF Andreas Pichlmair lab, Huang et al.91 N/A A549 OAS2 KO This paper N/A A549 NTC KO This paper N/A A549 OAS2 KO OAS2 WT KI cells This paper N/A A549 OAS2 KO OAS2 C652S KI cells This paper N/A A549 OAS2 KO OAS2 G2A KI cells This paper N/A A549 OAS2 KO OAS2 G2A C652S KI cells This paper N/A A549 OAS2 KO OAS2 F524L KI cells This paper N/A HeLa H1 ATCC Cat# CRL-1958 BJ HFF hTert+ Andreas Pichlmair, Huang et al.91 N/A Vero E6/TMPRSS2 Dr. Makoto Takeda, National Institute for Biological Standards and Control (NIBSC) Cat#100978 Experimental models: Organisms/strains Trichoplusia ni cells Expression Systems N/A Spodoptera frugiperda sf9 cells Expression Systems N/A Oligonucleotides See Table S2 N/A Recombinant DNA OAS plasmid constructs see Table S2 This paper N/A pET-28M-SUMO1-human OAS1 p46 This paper N/A pET-28M-SUMO1-human OAS1 p42 This paper N/A pACEBac1_N-6xHis_3C_hOAS3 p100 This paper N/A 2′5′-OA biosensor RNase L-Luciferase plasmid Alexei Korennykh, Chitrakar et al.62 N/A pACEBac1_N-6xHis_3C_hOAS2 p71 This paper N/A pACEBac1_N-6xHis_3C_hOAS2 p69 This paper N/A pACEBac1_N-6xHis_3C_hOAS2-C652S This paper N/A pACEBac1_N-6xHis_3C_hOAS2-p71 FL D153A S150A This paper N/A pLentiCRISPRv2 (hygromycin) Stringer et al. (2019)92 Addgene plasmid # 98291 pLIX403 (puromycin) David Root Addgene plasmid #41395 pLenti CMV/TO Zeo_GW (zeocin) Campeau et al. (2009)93 Addgene plasmid # 17294 pMD2-VSVG Andreas Pichlmair89 N/A pCMV-Gag-Pol Andreas Pichlmair89 N/A pCG_ACE2_IRES_eGFP Xie et al. (2023)94 N/A pCR XL TOPO OC43 N fragment This paper N/A pCR XL TOPO NL63 N fragment This paper N/A (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 85, 1–18.e1–e13, June 5, 2025 e3



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    plix403 puromycin david root addgene plasmid  (Addgene inc)
    96
    Addgene inc plix403 puromycin david root addgene plasmid
    Plix403 Puromycin David Root Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plix403+puromycin+david+root+addgene+plasmid/pLIX_403+(Plasmid+%2341395)/pm40412389-1017-272-276
    Average 96 stars, based on 1 article reviews
    plix403 puromycin david root addgene plasmid - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

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